1c114f61-5ee0-44ae-8bf1-22883dcc3e43 urn:uuid eng UTF-8 nonGeographicDataset nonGeographicDataset 2025-06-23T07:45:39 creation ISO 19115 2016 Marine eukaryotes and bacteria, Antarctica 1c114f61-5ee0-44ae-8bf1-22883dcc3e43 Glass slides were deployed at 12m and 18m3 at sites in McMurdo Sound (adjacent to McMurdo Station, Scott Base and Cape Armitage) for a period of 12 months to collect biofilm.18S rDNA sequence information was obtained for representatives of each DGGE (denaturing gradient gel electrophoresis) band at each site and depth. This data was interrogated to phyla level: cnidaria, bryozoa, protozoa, dinoflagellates, arthropods, platyhelminths and annelids were present.Density of diatoms observed by fluorescence microscopy for each site and depth were recorded (per 100 µm2). At least 6 different diatom morphotypes were identified based on size and morphology.The slides were also examined by performing fluorescence in situ hybridization (FISH) reactions with bacteria-specific probes (Eub338), an archaea-specific probe (ARCH915) and 9 different bacterial group-specific probes. Bacterial groups identified from the sequences were: Gammaproteobacteria, SRB, Planctomycetales, Alphaproteobacteria, Archaea, Betaproteobacteria, Actinobacteria and Firmicutes, Cytophaga /Flavobacteria of Bacteroidetes (CFB), Verrucomicrobia.This study was the first to examine eukaryotic recruitment, potentially overlooked in visual surveys, using molecular techniques. To use molecular techniques to examine the community structure of marine eukaryotes and bacteria recruiting onto artificial substrata. {"type": "Point", "coordinates": [166.6732, -77.863]} 166.6732 166.6732 -77.863 -77.863 Site-specific variation in Antarctic marine biofilms established on artificial surfaces: Webster NS and Negri AP (2006) Site-specific variation in Antarctic marine biofilms established on artificial surfaces. Environmental Microbiology 8: 1177-1190. documentDigital Site-specific variation in Antarctic marine biofilms established on artificial surfaces: Webster NS and Negri AP (2006) Site-specific variation in Antarctic marine biofilms established on artificial surfaces. Environmental Microbiology 8: 1177-1190. Recruitment of Antarctic marine eukaryotes onto artificial surfaces: Webster NS, Battershill CN and Negri AP (2006) Recruitment of Antarctic marine eukaryotes onto artificial surfaces. Polar Biology 30: 1-10. documentDigital Recruitment of Antarctic marine eukaryotes onto artificial surfaces: Webster NS, Battershill CN and Negri AP (2006) Recruitment of Antarctic marine eukaryotes onto artificial surfaces. Polar Biology 30: 1-10. AIMS Web Site documentDigital audio/basic AIMS Web Site documentDigital audio/basic AIMS Web Site documentDigital audio/basic AIMS Web Site documentDigital audio/basic Unnamed resource documentDigital License Graphic PNG Unnamed resource documentDigital HTML