19d3b273-131a-44ac-b89e-cd2fa1d4e9a2 urn:uuid eng UTF-8 nonGeographicDataset nonGeographicDataset 2025-06-23T06:48:51 creation ISO 19115 2016 Euphausiid-specific metabarcoding data from eDNA samples collected on the TEMPO voyage 19d3b273-131a-44ac-b89e-cd2fa1d4e9a2 This is a Euphausiid-specific metabarcoding dataset of Southern Ocean eDNA samples collected on the TEMPO voyage in 2021. We collected eDNA samples by filtering five litres of seawater in the East Antarctic Southern Ocean from the surface and seafloor as well as in and near krill swarms(n = 110). After DNA extraction, a Euphausiid-specific metabarcoding marker was used to determine the presence of different Southern Ocean krill species in each sample. Detailed methods are available in the following publication: Suter L et al. (2025) Monitoring Antarctic krill (Euphausia superba) distribution in the Southern Ocean: environmental DNA (eDNA) adds to the toolbox. Frontiers in Marine Science. The data provided here contains - raw paired end sequencing data in fastq format for each sample - R markdown code to process the raw data in order to identify unique sequences, and which sequences are contained in which samples - a tag file that identifies which multiplex-identifier (MID) tags were used in forward and reverse primers for each sample - a zOTU table that identifies which sequences were detected in each sample, and what taxonomy was associated with each sequence. {"type": "Polygon", "coordinates": [[[55.0, -68.0], [80.0, -68.0], [80.0, -62.83], [55.0, -62.83], [55.0, -68.0]]]} 55.0 80.0 -68.0 -62.83 ADS > Automated DNA Sequencer AMD AMD/AU CEOS EARTH SCIENCE > BIOLOGICAL CLASSIFICATION > ANIMALS/INVERTEBRATES > ARTHROPODS > CRUSTACEANS > EUPHA EARTH SCIENCE > BIOSPHERE > ECOLOGICAL DYNAMICS > SPECIES/POPULATION INTERACTIONS > INDIGENOUS/NATIV GEOGRAPHIC REGION > POLAR METABARCODING OCEAN > SOUTHERN OCEAN OCEAN > SOUTHERN OCEAN > EAST ANTARCTIC CONTINENTAL SHELF R/V INVESTIGATOR Ships TEMPO GET DATA > DIRECT DOWNLOAD documentDigital Download the dataset. HTML PROJECT HOME PAGE documentDigital Public information for AAS project AAS_4556 HTML VIEW RELATED INFORMATION documentDigital Citation reference for this metadata record and dataset. HTML Creative Commons by Attribution logo documentDigital PNG