26b98dca-38e5-4b29-b9a9-a866eef196f1 urn:uuid eng UTF-8 nonGeographicDataset nonGeographicDataset 2025-06-23T15:47:10 creation ISO 19115 2016 RNA sequence data from krill CO2 exposure experiment 26b98dca-38e5-4b29-b9a9-a866eef196f1 We use RNA sequencing to investigate which genetic/physiological pathways in Antarctic krill are affected by increased CO2 levels. We carried out larval CO2 exposure experiments in March 2012 at the AAD aquarium. Two developmental stages were used (Calyptopis I and Furcilia V) and three CO2 levels (control, 1000 and 2000 ppm). These were short term experiments (2 days) - since initial longer experiments starting with fertilized eggs resulted in differences in developmental stages between treatments and control which could confound the data. RNA was extracted from larvae and high-throughput RNA sequencing (RNA-seq) was carried out on 6 samples (2 stages * 3 treatments). Sequencing was carried out on an Illumina sequencer (Genome Analyzer II). We collected ~ 60 million sequence reads per sample (Data in FASTA format each read gives 100 base pairs of sequence), so a total of ~360 million reads (36 billion bp of data). {"type": "Polygon", "coordinates": [[[147.29014, -42.98792], [147.29396, -42.98792], [147.29396, -42.9855], [147.29014, -42.9855], [147.29014, -42.98792]]]} 147.29014 147.29396 -42.98792 -42.9855 ADS > Automated DNA Sequencer AMD AMD/AU CEOS CONTINENT > ANTARCTICA CONTINENT > AUSTRALIA/NEW ZEALAND > AUSTRALIA EARTH SCIENCE > BIOLOGICAL CLASSIFICATION > ANIMALS/INVERTEBRATES > ARTHROPODS > CRUSTACEANS > EUPHA EARTH SCIENCE > OCEANS > OCEAN CHEMISTRY > CARBON DIOXIDE GENETICS GEOGRAPHIC REGION > POLAR LABORATORY OCEAN > SOUTHERN OCEAN OCEAN ACIDIFICATION RNA GET DATA documentDigital Request point for the data HTML PROJECT HOME PAGE documentDigital Public information for AAS project AAS_4015 HTML VIEW RELATED INFORMATION documentDigital Citation reference for this metadata record and dataset. HTML Creative Commons by Attribution logo documentDigital PNG