From Australian Oceans Data Network
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Responses of ambon damselfish, a common Great Barrier Reef species, to the organophosphorus insecticide, chlorpyrifos

Created 24/06/2025

Updated 24/06/2025

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Pomacentrus amboinensis embryos were exposed experimentally, to a range of concentrations of the organophosphorus insecticide chlorpyrifos and monitored for survival and sublethal effects. Eggs from a single clutch were obtained from the captive breeding program at James Cook University and transferred to aquaria at the Australian Institute of Marine Science. Appropriate amounts of chlorpyrifos were added to 50 µL of acetone and 700 ml of 1 µm filtered seawater to produce treatment solutions with concentrations of 50, 125, 250, 500, 750, 1000, 1250, 2000 µg/L. A control, which contained 50 µL of acetone in seawater was also used. Water quality parameters were monitored throughout the experiments (dissolved oxygen >85% saturation, pH 8.1±0.1, temperature 27±1°C, salinity 33.3±0.2 0/00).Toxicity tests were carried out using healthy fertilised eggs, less than 10 hours old. Twenty randomly selected eggs were placed in each of three replicate 900 ml treatment chambers for each chlorpyrifos concentration. Numbers of dead, immobilised or abnormal embryos were counted after 72 and 120 hours and dead embryos removed. Heart rates were measured over a 1 minute period for 5 embryos from each treatment after 96 hours, using a Hi-8 video system connected to a stereo dissecting microscope.On completion of hatching, numbers of normal, dead, or deformed larvae were counted and the percentage viable hatch calculated for each replicate. Surviving larvae were killed and their standard lengths measured. The experiment was terminated at 144 hours, as all eggs had hatched and mortality was extremely high at the highest concentrations. This research was undertaken to assess the potential for use of the ambon damselfish (Pomacentrus amboinensis), a widespread and abundant reef fish species, as a potential bioindicator of the effects of toxic contaminants in Great Barrier Reef waters. The organophosphorus insecticide chlorpyrifos was selected for testing, as it is the most widely used insecticide in the sugar cane growing regions of Queensland.

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Additional Info

Field Value
Title Responses of ambon damselfish, a common Great Barrier Reef species, to the organophosphorus insecticide, chlorpyrifos
Language eng
Licence Not Specified
Landing Page https://data.gov.au/data/dataset/a0cbdc54-d452-403a-9c66-bc74ed3467cb
Contact Point
Australian Ocean Data Network
reception@aims.gov.au
Reference Period 20/11/2017
Geospatial Coverage Australia
Data Portal Australian Oceans Data Network

Data Source

This dataset was originally found on Australian Oceans Data Network "Responses of ambon damselfish, a common Great Barrier Reef species, to the organophosphorus insecticide, chlorpyrifos". Please visit the source to access the original metadata of the dataset:
https://catalogue.aodn.org.au/geonetwork/srv/eng/csw/dataset/responses-of-ambon-damselfish-a-common-great-barrier-reef-species-to-the-organophosphorus-insec1