From Australian Oceans Data Network
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Spatial metabolomics for symbiotic marine invertebrates

Created 23/06/2025

Updated 23/06/2025

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A mass spectrometry imaging workflow was developed for a soft- and hard-bodied cnidarian animal capable of revealing the host and symbiont metabolome in situ, without the need for a priori isotopic labelling or skeleton decalcification. Through the combination of anaesthetizing, embedding, and cryofilm application, the cryosectioning workflow presented produced intact tissue sections for a soft-bodied (sea anemone E. diaphana) and a hard-bodied (coral G. fascicularis) cnidarian in their natural shape for MSI metabolomics or spatial metabolomics. The study used: Soft bodied cnidarians sourced from the GBR - E. diaphana (genotype AIMS4) in symbiosis with the homologous Breviolum minutum (hereafter referred to as B1-anemones, which were inoculated with symbiont culture SCF 127-01) or the heterologous Cladocopium proliferum (C1-anemones) were inoculated with symbiont culture SCF 055-01.10 E. diaphana were sampled 10 mo post-inoculation for MSI and were maintained under ambient temperature of 27°C at 30 μmol m−2 s−1 (12:12 h, light: dark) Hard-bodied cnidarians, the coral G. fascicularis in symbiosis with Cladocopium C40 (ITS2 profile: C40-C3-C115-C40h) was obtained from Palm Islands GBR and kept under ambient temperature of 27°C and at 130–150 μmol m−2 s−1 at full sun (12:12 h light: dark) ITS2 profiles of the symbiont cultures: culture SCF 127-01 - ITS2 profile: B1-B1o-B1p culture SCF 055-01.10 - ITS2 profile: C1-C1b-C1c-C42.2-C1br-C1bh-C1cb-C72k Cladocopium C40: ITS2 profile: C40-C3-C115-C40h

Files and APIs

Additional Info

Field Value
Title Spatial metabolomics for symbiotic marine invertebrates
Language eng
Licence Not Specified
Landing Page https://data.gov.au/data/dataset/20d577ae-d5bb-4073-b999-dbea160a305d
Contact Point
Australian Ocean Data Network
reception@aims.gov.au
Reference Period 08/03/2024
Geospatial Coverage
Map data © OpenStreetMap contributors
{
  "coordinates": [
    147.05520744774122,
    -19.26720760708421
  ],
  "type": "Point"
}
Data Portal Australian Oceans Data Network

Data Source

This dataset was originally found on Australian Oceans Data Network "Spatial metabolomics for symbiotic marine invertebrates". Please visit the source to access the original metadata of the dataset:
https://catalogue.aodn.org.au/geonetwork/srv/eng/csw/dataset/spatial-metabolomics-for-symbiotic-marine-invertebrates